Examination of the Long-range Effects of Aminofluorene-induced Conformational Heterogeneity and Its Relevance to the Mechanism of Translesional DNA Synthesis

Adduct-induced conformational heterogeneity complicates the understanding of how DNA adducts exert mutation. A case in point is the N-deacetylated AF lesion [N-(2′-deoxyguanosin-8-yl)-2-aminofluorene], the major adduct derived from the strong liver carcinogen N-acetyl-2-aminofluorene. Three conformational families have been previously characterized and are dependent on the positioning of the aminofluorene rings: B is in the “B-DNA” major groove, S is “stacked” into the helix with base-displacement, and W is “wedged” into the minor groove. Here, we conducted 19F NMR, CD, Tm, and modeling experiments at various primer positions with respect to a template modified by a fluorine tagged AF-adduct (FAF). In the first set, the FAF-G was paired with C and in the second set it was paired with A. The FAF-G:C oligonucleotides were found to preferentially adopt the B or S-conformers while the FAF-G:A mismatch ones preferred the B and W-conformers. The conformational preferences of both series were dependent on temperature and complementary strand length; the largest differences in conformation were displayed at lower temperatures. The CD and Tm results are in general agreement with the NMR data. Molecular modeling indicated that the aminofluorene moiety in the minor groove of the W-conformer would impose a steric clash with the tight-packing amino acid residues on the DNA binding area of the Bacillus fragment (BF), a replicative DNA polymerase. In the case of the B-type conformer, the carcinogenic moiety resides in the solvent-exposed major groove throughout the replication/translocation process. The present dynamic NMR results, combined with previous primer extension kinetic data by Miller & Grollman, support a model in which adduct-induced conformational heterogeneities at positions remote from the replication fork affect polymerase function through a long-range DNA–protein interaction

CONTRIBUTE, DETACH AND AUTONOMOUS ACCESS TO CIPHER TEXT IN CLOUD DATABASE

In some approaches to repository functions have been adopted, while the approach to building trust in the message as a taboo of interests is being developed to be carefully dealt with. Having a pulse to swallow an embarrassing information usage ensures the withdrawal and implements the appropriate procedure in the encrypted message. Introducing the information, you need to distract yourself must confirm your trust, as well as an easy approach to the message. The car builds on popular encryption programs as well as unique ways to tune the encrypted data in hard to believe information into the shower. Secure confidential information acts as a basic job of creating a hacker to distract and discern irresponsibility, in addition to the gain measurement options, loss of unencrypted messages pointing to deformation Temporary and offer many options they detect the best way for tape information functions. The solid information prediction function does not retain anything legally right before the information has been previously benefited; it does not need any suggestion for the distraction function of the command

A New Hybrid Root-Finding Algorithm to Solve Transcendental Equations Using Arcsine Function

The objective of this paper is to propose a new hybrid root finding algorithms for solving non-linear equations (NLEs) or transcendental equations (TEs). The proposed algorithm is based on the trigonometrical algorithm using arcsine function to find a root. Several numerical examples are presented to illustrate the proposed algorithms, and comparisons are presented with other existing methods to show efficiency and accuracy. Implementation of the proposed algorithms is presented in a mathematical software tool Maple

Large spontaneous exchange bias in a weak ferromagnet Pb6Ni9(TeO6)5

We report the magnetic and dielectric behavior of Pb6Ni9(TeO6)5, a new compound comprising the honeycomb-like layers of S=1 spins, through detailed structural, magnetic and dielectric investigation. An antiferromagnetic-type transition at 25 K (TN) with weak-ferromagnetic behavior is revealed. Interestingly, a large value of coercive field of 1.32 T at 2 K is observed. The isothermal magnetization after zero-field-cooled condition, it exhibits the presence of large spontaneous exchange bias (SEB) with a magnitude of 0.19 T at 2 K; which is rare in single bulk materials, especially without external doping. The value of |HEB| further enhances to 0.24 T under 16 T field-cooled condition, confirming the presence of large exchange bias in the material. In addition, the dielectric constant shows an anomaly at the onset of TN, indicating the presence of magnetodielectric coupling.Comment: 16 pages, 6 figures, 2 supplimentory figures, under revision of Scientific Report

Frequency stabilization in interconnected power system using bat and harmony search algorithm with coordinated controllers

Modern power system faces excessive frequency aberrations due to the intermittent renewable generations and persistently changing load demands. To avoid any possible blackout, an efficient and robust control strategy is obligatory to minimize deviations in the system frequency and tieline. Hence, to achieve this target, a new two-degree of freedom-tilted integral derivative with filter (2DOF–TIDN) controller is proposed in this work for a two-area wind-hydro-diesel power system. To enhance the outcome of the proposed 2DOF–TIDN controller, its gain parameters are optimized with the use of a newly designed hybrid bat algorithm-harmony search algorithm (hybrid BA–HSA) technique. The effectiveness and superiority of hybrid BA–HSA tuned 2DOF–TIDN is validated over various existing optimization techniques like cuckoo search (CS), particle swarm optimization (PSO),HSA, BA and teaching learning-based optimization (TLBO). To further refine the system outcome in the dynamic conditions, several flexible AC transmission systems (FACTS) and superconducting magnetic energy storage (SMES) units are adopted for enriching the frequency and tie-line responses. The FACTS controllers like static synchronous series compensator (SSSC), thyristor-controlled phase shifter (TCPS), unified power flow controller (UPFC) and interline power flow controller (IPFC) are employed with SMES simultaneously. The simulation results disclose that the hybrid BA–HSA based 2DOF–TIDN shows superior dynamic performance with IPFC–SMES than other studied approaches. A sensitivity analysis is examined to verify the robustness of proposed controller under ±25% changes in loading and system parameters

Vertex Degrees and Isomorphic Properties in Complement of an m

Computational intelligence and computer science rely on graph theory to solve combinatorial problems. Normal product and tensor product of an m-polar fuzzy graph have been introduced in this article. Degrees of vertices in various product graphs, like Cartesian product, composition, tensor product, and normal product, have been computed. Complement and μ-complement of an m-polar fuzzy graph are defined and some properties are studied. An application of an m-polar fuzzy graph is also presented in this article

Morphism of m

The main purpose of this paper is to introduce the notion of m-polar h-morphism on m-polar fuzzy graphs. The action of m-polar h-morphism on m-polar fuzzy graphs is studied. Some elegant theorems on weak and coweak isomorphism are obtained. Also, some properties of highly irregular, edge regular, and totally edge regular m-polar fuzzy graphs are studied

Microbial properties of soils as affected by cropping and nutrient management practices in several long-term manurial experiments in the semi-arid tropics of India

Microorganisms play a critical role in nutrient transformation, soil health and for sustaining the productivity of soils. Effects of long-term cropping, fertilization, manuring and their integration on microbial community were studied in soil samples from five long-term fertilizer experiments under various rainfed production systems in the semi-arid tropics (SAT) of India. Microbial population counts were analyzed by dilution plating and were in turn compared with different parameters such as soil treatments, soil type, soil microbial biomass C, soil organic C, rainfall and soil pH. The counts were high in treatments where combinations of organic and inorganic fertilizers were applied compared to control. Vertisols showed larger organic carbon levels than Alfisols. Fungal population was higher in acidic soils and in treatments under continuous inorganic fertilization treatments whereas a high number of bacteria were found in integrated use of organic and inorganic fertilizers. At most of the locations soil organic C and microbial biomass C showed significant positive ( p � 0.05) correlation with microbial populations. Thus, results suggest that even under arid and semi-arid tropical conditions, regular addition of nutrients in an integrated manner could improve soil organic carbon and microbial population counts. For each production system, better carbon sequestration management practices were identifie

Performance analysis of distributed power flow controller with ultra-capacitor for regulating the frequency deviations in restructured power system

This paper presents a novel approach for automatic generation control (AGC) of two-area deregulated system with unequal sources for sustaining the frequency and tie-line power at perturbations. The combination of ultra-capacitor (UC) and various FACTS controllers such as Thyristor-controlled series capacitor (TCSC), Static synchronous series compensator (SSSC), Unified power flow controller (UPFC), and Distributed power flow controller (DPFC) have been investigated in AGC of interconnected system with thermal-wind and hydro-diesel generating units. An innovative metaheuristic method called bat algorithm (BA) is used to ascertain the optimal gain parameters of the two degree of freedom (2DOF) controllers using an integral squared error (ISE) criteria. Furthermore, the productive assessment of the bat tuned 2DOF controllers are also compared with teaching learning-based optimization (TLBO) and cuckoo search (CS) methods optimized 2DOF in distinct contract scenarios of the suggested restructured system. The effect of the coordinated performance of UC and DPFC has been mitigated the oscillatory response of the AGC system at various operating circumstances. The investigations disclose that the bat optimized 2DOF-PID yield the productive outcomes with coordination of DPFC and UC in all contract transactions of the restructured system